Welcome to the Lemieux Lab     

We are a discovery research lab that focuses on understanding the function of proteins in health and disease. Our lab focuses on the structure of function membrane proteins with a particular focus on:


1. Intramembrane proteolysis and neurodegenerative disease

https://www.ncbi.nlm.nih.gov/pubmed/?term=lemieux+rhomboid


2. Enzymes involved in lipid production in humans and plants (Food science, cardivascular disease)

https://www.ncbi.nlm.nih.gov/pubmed/?term=lemieux+DGAT1


3. Gradient-driven transporters

https://www.ncbi.nlm.nih.gov/pubmed/?term=Lemieux+MJ%5BAuthor%5D+transport


4. Optogenetics (biosensors)

to come


5. Immune checkpoint receptors (Cancer research)

https://www.ncbi.nlm.nih.gov/pubmed/?term=lemieux+barakat


Sponsorships:

Sponsor a trainee. Contact us for more details.

joanne.lemieux”@“ualberta.ca


Affiliations:

University of Alberta:

Neuroscience and Mental Health Insitute

Women and Children’s Health Research Institute

Cancer Research Institute of North America

Phytola


International:

International Proteolysis Society

Biophysical Society


RESEARCH FOCUS


1. RHOMBOID PROTEASES

Our lab focuses on the structure and function of intramembrane proteases such as those belonging to the rhomboid peptidases family. These are membrane embedded proteases that cleave membrane proteins. (Lemieux et al., PNAS, 2007, 2NR9.pdb; Brooks et al., J. Mol. Biol. 2011). We use a combination of techniques including X-ray crystallography and biochemistry to assess protein structure and function.


Rhomboid

hiGlpG rhomboid (pdb code 2NR9)


2. EXPRESSION OF EUKARYOTIC TARGETS

Our lab is also focusing on strategies to enhance expression of several eukaryotic membrane protein targets. Using GFP-tagged targets, we have developed a plate-based screen to assess expression levels of colonies prior to cell culture (Brooks et. al., Protein Science 2014).


3. MEMBRANE-EMBEDDED ENZYMES INVOLVED IN LIPID PRODUCTION

Our lab has worked on expression of several enzymes involved in lipid production. See: Brooks et. al., Protein Science 2014, and Caldo et al, FEBS Lett. 2015.

© University of Alberta 2012